Fig. 4: Chaperones correct NCLE misfolding bias in essential but not non-essential proteins. | Nature Communications

Fig. 4: Chaperones correct NCLE misfolding bias in essential but not non-essential proteins.

From: A widespread protein misfolding mechanism is differentially rescued in vitro by chaperones based on gene essentiality

Fig. 4

(left) odds ratio between native NCLE regions and presence of significant cut-sites (misfolding) for the set of essential (blue, \({n}_{{{{\rm{proteins}}}}}=81\), \({n}_{{{{\rm{residues}}}}}=27,709\)) and non-essential (red, \({n}_{{{{\rm{proteins}}}}}=183\), \({n}_{{{{\rm{residues}}}}}=60,007\)) proteins containing native NCLE in the absence of chaperones determined by logistic regression. ***, **, * indicate, respectively, conditions in which the two-sided \(p\) values are below significance thresholds of 0.001, 0.01, and 0.05. (middle), same but in the presence of DnaK/J for essential (\({n}_{{{{\rm{proteins}}}}}=44\), \({n}_{{{{\rm{residues}}}}}=13,617\)), and non-essential (\({n}_{{{{\rm{proteins}}}}}=97\), \({n}_{{{{\rm{residues}}}}}=31,112\)). (right), same but in the presence of GroEL/ES for essential (\({n}_{{{{\rm{proteins}}}}}=55\), \({n}_{{{{\rm{residues}}}}}=18,667\)), and non-essential (\({n}_{{{{\rm{proteins}}}}}=137\), \({n}_{{{{\rm{residues}}}}}=43,593\)). The raw data used to make these plots were used to calculate the change in the OR results and the significance of the change beyond random chance by permutation testing. Data are presented as mean values with 95% confidence interval.

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