Table 2 Recommended conditions for the fluorescent assay to test modification of RoCKseq beads

From: RoCK and ROI: single-cell transcriptomics with multiplexed enrichment of selected transcripts and region-specific sequencing

Condition

Beads

Fluorescent oligo

Positive control dT

Barcoded beads (unmod)

polyA fluo oligo

Positive control TSO

Barcoded beads (unmod)

TSO fluo oligo

Negative control

Barcoded beads (unmod)

Fluo oligo for modification

RoCKseq beads

Barcoded beads (mod)

Fluo oligo for modification

dT control RoCKseq beads

Barcoded beads (mod)

polyA fluo oligo

Unmodified beads

Barcoded beads

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