Fig. 5: Robust engagement of LIG3α with an undamaged 601 NCP at the single molecule level.
From: The zinc finger of DNA ligase 3α binds to nucleosomes via an arginine anchor
a A corresponding kymograph of an observation for HaloTag-635-LIG3α-XRCC1-YFP binding undamaged 601 NCP at 5 pN tension. b Cumulative residence time distribution (CRTD) and cumulative gap time distribution (CGTD), analysis for HaloTag-635-LIG3α binding undamaged 601 NCP, with a single exponential fit shown in red. c Cumulative residence time distribution (CRTD) and cumulative gap time distribution (CGTD), analysis for XRCC1-YFP binding, undamaged 601 NCP with a single exponential fit shown in blue. d The distribution of the 11 categories for HaloTag-635-LIG3α and XRCC1-YFP binding to undamaged 601 NCP. Error bars represent the SEM of two experiments. e A domain model of ZnF-BRCT LIG3α construct, followed by a resultant 2D scan demonstrating co-localization between the Cy3 signal of the nucleosome and the ATTO 647 N dye (represented by yellow). f A corresponding kymograph of an observation for HaloTag-635-ZnF-BRCT LIG3α-XRCC1-YFP binding undamaged 601 NCP at 5 pN tension. g Cumulative residence time distribution (CRTD) and cumulative gap time distribution (CGTD), analysis for HaloTag-635-ZnF-BRCT LIG3α, with a two-phase exponential fit shown in red. h Cumulative residence time distribution (CRTD) and cumulative gap time distribution (CGTD), analysis for XRCC1-YFP, with a two-phase exponential fit shown in blue. i The distribution of the 11 categories for HaloTag-635-ZnF-BRCT LIG3α and XRCC1-YFP binding to a nondamaged NCP. Error bars represent the SEM of two experiments. Created in BioRender. Gupta, A. (2025) https://BioRender.com/x8z0yih. Source data are provided as a Source Data file.
