Fig. 6: Positioning of non-ligatable nicks within the 601NCP alters LIG3α and XRCC1 binding kinetics.
From: The zinc finger of DNA ligase 3α binds to nucleosomes via an arginine anchor
a Structural model of the nucleosomes generated from PDB 4JJN, with sites of each nick site marked along the structure (with SHL0, SHL-2.5, and SHL−4.5). b A corresponding kymograph of an observation for HaloTag-635- LIG3α-XRCC1-YFP binding 601 NCP having the nick at SHL –4.5 position, at 5 pN tension, along with the percentage of colocalized events and binding kinetics (see Table 2). c A corresponding kymograph of an observation for HaloTag-635- LIG3α-XRCC1-YFP binding 601 NCP having the nick at SHL -2.5 position, at 5 pN tension, along with the percentage of colocalized events and binding kinetics (see Table 2). d A corresponding kymograph of an observation for HaloTag-635- LIG3α-XRCC1-YFP binding 601 NCP having the nick at SHL 0 position, at 5 pN tension, along with the percentage of colocalized events and binding kinetics (see Table 2). Created in BioRender. Gupta, A. (2025) https://BioRender.com/ray3txr. Source data are provided as a Source Data file.
