Fig. 4: Kap dynamics are described by FG motif density and available space (steric hindrance).

a Position of a selected Kap along the pore axis as a function of time. The Kap does not translocate in one go, rather, it dwells at several locations in the pore (red time intervals). b Displacement of the selected Kap as a function of time, where the displacement is measured over a time window of 250 μs. c Number of FG-interactions that the selected Kap experiences as a function of time. d Contacts per FG-Nup type. e Selected simulation snapshots of the selected Kap overlaid on top of the FG motif distribution (top row) and the total protein distribution of both FG-Nups and Kaps (bottom row). Each snapshot represents an averaged density projection over a 20 nm thick slice of the simulation volume, centered on the Kap and taken in the x–z plane (in contrast to the r–z projections used elsewhere). The complete translocation event is shown in the Suppl. Movie S2. f–h Trajectory of the selected Kap shown on top of the average density distribution of the GLFG-Nups. The red segments of the trajectory correspond to the time intervals highlighted in (a–d). The trajectory is projected onto the f x–z, g y–z, and h x–y planes.