Fig. 5: XL-MS validates features of Augmin hetero-tetramer, Augmin hetero-octamer, and antiparallel Augmin dimer. | Nature Communications

Fig. 5: XL-MS validates features of Augmin hetero-tetramer, Augmin hetero-octamer, and antiparallel Augmin dimer.

From: Cryo-EM structures of plant Augmin reveal coiled-coil assembly, antiparallel dimerization, and NEDD1 binding

Fig. 5: XL-MS validates features of Augmin hetero-tetramer, Augmin hetero-octamer, and antiparallel Augmin dimer.

A Crosslinks identified in AUG1,3,4,5, illustrated in the context of the cryo-EM structural model of AUG1,3,4,5. In total, 216 unique residue pairs were identified, of which the 122 with Cα-Cα distances less than 35 Å are displayed. B Crosslinks identified in AUG1,2,3,4,5,6,7,8 illustrated in the context of the cryo-EM structural model of AUG1,2,3,4,5,6,7,8. In total, 115 unique residue pairs were identified, of which the 85 with Cα-Cα distances less than 35 Å are displayed. C Crosslinks identified in the AUG1,2,3,4,5,6,7,8 illustrated in the context of the cryo-EM structural model of the anti-parallel Augmin dimer. The 32 crosslinks which have shorter Cα-Cα distances in the context of the anti-parallel dimer compared to the monomer are shown.

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