Fig. 7: Coevolutionary analysis and direct coupling analyses support the Augmin hetero-octameric assembly structure and its interface with NEDD1-WD β propeller.

A Circular summary of eukaryotes with Augmin subunit(s) and NEDD1. Concentric circles encode taxonomy (center—kingdom; middle—class. The outer rim annotates conservation of the eight Augmin subunits and NEDD1 across species (pink—conservation; light cyan—absence of one or more subunits). A complete evolutionary tree is shown in Supplementary Fig. 15A. B Line-based co-evolutionary representation of Augmin subunits and NEDD1 across eukaryotes. Each line represents a unique species. Line color code: yellow—protein is absent; green—protein is present; purple—complete set (all eight Augmin subunits plus NEDD1 in that species). C Structural model of the Augmin hetero-octamer displaying DCA pairs as bars across Augmin subunits. Pairs are colored by significance (red: p ≤ 0.05, blue: 0.05 <p ≤ 0.1; black: 0.1 <p ≤ 0.2). The p-values were calculated using right tailed Hypergeometric test, testing for enrichment of true positive inter-domain DCA contacts among a given number of predicted DI pairs. Full pair list is in Supplementary Table 3. D Circular view of the sequences in Augmin subunits with the DCA pairs colored as in (C). E Augmin hetero-octamer V-junction model displaying the NEDD1-WD β-propeller and its DCA pairing (black bars) to AUG2,5,6,8, as detailed in Supplementary Table 5.