Fig. 4: Depletion of H3K36me induces changes in DNA methylation, H3K4me1 and H3K9me3 within gene bodies.
From: H3K36 Methylation as a Guardian of Epigenome Integrity
a Metagene plots of DNA methylation within gene bodies (same groups of genes used in Fig. 3), illustrating a decrease in DNA methylation in unexpressed and lowly expressed genes. b Metagene plots indicating increasing H3K4me1 within gene bodies following depletion of H3K36me. c Barplot illustrating the increasing global abundance of H3K4me1, as determined by mass spectrometry (MS), following successive deletions of K36MTs. d Metagene plots indicating invasion of H3K9me3 within gene bodies following depletion of H3K36me2 in DKO and TKO cell lines. e Barplot derived from MS, showing that genome-wide H3K9me3 abundance does not significantly change across KO cell lines. For b and d, CUT&RUN or ChIP-seq signals, respectively, were MS-normalized as previously described. For c and e, error bars display the standard deviation around the mean. (n = 3 per condition). Source data are provided as a Source Data file.
