Fig. 5: Enzymatic deglycosylation by endo H treatment destabilizing Sing/16 HA. | Nature Communications

Fig. 5: Enzymatic deglycosylation by endo H treatment destabilizing Sing/16 HA.

From: Structural and immunological characterization of the H3 influenza hemagglutinin during antigenic drift

Fig. 5: Enzymatic deglycosylation by endo H treatment destabilizing Sing/16 HA.

A Cryo-EM 3-D classification results of HK/68 and Sing/16 HA after treatment with endo H, and (B) the resulting 3-D refinement of the selected and pooled classes. Also shown are percentages of retained and discarded particles and an overlay of 3-D reconstructions representing the two extremes of the 1st principal component from 3-D variability analysis in CryoSparc. C Glycan difference map of HK/68 and Sing/16. D Normalized glycan mean intensity for untreated HA (blue) and endo-H treated HA (orange). E Glycan occupancy around the third glycan residue, β-mannose (BMA) between the GnTI- and stable endo H treated 3-D reconstructions of HK/68 and Sing/16. F Thermostability of both HA variants obtained from nano-DSF measurements. Data was plotted using ChimeraX and Prism 9. Source data are provided as a Source Data file.

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