Fig. 7: Disruption of histone acetylation homeodynamics attenuates tumorigenesis in Drosophila and mice.
From: Histone acetylation homeodynamics navigates cell survival and apoptosis
a, b’ Evaluation of tumorigenesis after CtBP-i, HDAC3-i, and Tip60-i in RasV12 (a) or Yki3SA (b) overexpression-induced tumors in Drosophila wing discs using Flp-out line, with statistical analysis of the tumor size in a (a’) and b (b’). Tumor cells were labelled by GFP. Data are presented as mean ± SD. Two-tailed paired t test: ***p < 0.001. In a’, n = 8, 8, 8, 8 independent wing disc. In b’, n = 9, 8, 9, 8 independent wing disc. c, d’ Evaluation of tumorigenesis after CtBP-i, HDAC3-i, and Tip60-i in scrib-i (c) or lgl2-i (d)-induced tumors between the two domains of the dorsal medial fold in Drosophila wing discs using upd-Gal4, with statistical analysis of the tumor size in c (c’) and d (d’). The two domains of the dorsal medial fold were labelled by GFP, and the tumor location was marked by red arrows. Data are presented as mean ± SD. Two-tailed paired t test: *p < 0.05, ***p < 0.001. In c’, n = 22, 29, 27, 27 independent wing disc. In d’, n = 15, 16, 8, 10 independent wing disc. e–g After subcutaneous injection of stable HomoCtBP, HomoHDAC3, HomoCREBBP, and HomoKAT5 knockdown A549 cells, Scramble knockdown A549 cells was used as the control group. The mean tumor volume in each group before sacrifice (e), mean ± SEM. n = 6 independent mice. Images (f) and wet weights (g) of the tumors from each group after sacrifice, mean ± SD; n = 6 independent mice. Two-tailed paired t test: *p < 0.05, **p < 0.01. In a’-d’ and g, box plots display the distribution of tumor size and wet weights. The top and bottom of the box represent the 75th (Q3) and 25th (Q1) percentiles, respectively. The line inside the box indicates the median. The whiskers extend to the farthest data points within 1.5× interquartile range (IQR) from the quartiles, and points beyond the whiskers are defined as outliers and plotted as individual dots. Source data are provided as a Source Data file. The genotypes are provided in Supplementary Table 3.
