Fig. 1: Cryo-EM structures of the SAM complex purified in detergent reveal a dynamic lateral gate. | Nature Communications

Fig. 1: Cryo-EM structures of the SAM complex purified in detergent reveal a dynamic lateral gate.

From: The dynamic lateral gate of the mitochondrial β-barrel biogenesis machinery is blocked by darobactin A

Fig. 1: Cryo-EM structures of the SAM complex purified in detergent reveal a dynamic lateral gate.The alternative text for this image may have been generated using AI.

Cryo-EM maps of the T. thermophilus SAM complex colored by subunit (Sam50 in teal, Sam37 in yellow, Sam35 in green, density attributed to lipid or detergent molecules in gray). A SAMcl density front and side view. B SAMop density front and side view. (C) SAMcl structure front and side view. D SAMop structure front and side view. E Superposition of SAMcl (blue) and SAMop (pink) generated using ChimeraX v1.4 matchmaker86,99. Lateral gate region shown in zoom inset. F–G MD simulations reveal a Sam50 β-barrel in a stable closed conformation. β1 and β16 are connected with, on average, three hydrogen bonds. F Average number of hydrogen bonds between the β1 and β16 strands of Sam50 for the last 2 μs of each simulation. Each data point represents a single replica. G Sam50 in a zipped closed conformation, observed with, at most, a couple of hydrogen bonds (black dotted lines). See also Supplementary Figs. 5, 6. Source data are provided as a Source Data file.

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