Fig. 3: Darobactin A inhibits mitochondrial β-barrel biogenesis in vitro. | Nature Communications

Fig. 3: Darobactin A inhibits mitochondrial β-barrel biogenesis in vitro.

From: The dynamic lateral gate of the mitochondrial β-barrel biogenesis machinery is blocked by darobactin A

Fig. 3: Darobactin A inhibits mitochondrial β-barrel biogenesis in vitro.

Isolated wild-type S. cerevisiae mitochondria were incubated with increasing concentrations of darobactin A prior to incubation with translation lysate containing radiolabeled S. cerevisiae (A) Tom40, (B) Mdm10, (C) Porin1, or (D) Sam50. Following incubation, mitochondrial membranes were solubilized with digitonin, soluble fraction was subjected to BN-PAGE and autoradiography was used to identify protein complexes containing radiolabeled proteins. Data are representative of two (Sam50, Porin1) to three (Tom40, Mdm10) independent experiments. Replicate data and source data are provided as a Source Data file.

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