Table 1 Cryo-EM data collection, structure determination, and model statistics
SAMop | SAMcl | SAMdaro | |
|---|---|---|---|
Data collection | |||
Nominal magnification | 105,000x | 105,000x | |
Voltage (kV) | 300 | 300 | |
Exposure time (s/frame) | 0.05 | 0.05 | |
Number of frames | 40 | 50 | |
Total dose (e-/Å2) | 70.0 | 54.4 | |
Defocus range (μm) | −0.6 to −1.6 | −0.7 to −2.0 | |
Pixel size (Å) | 0.412/super-res pix | 0.415/super-res pix | |
Image processing | |||
Micrographs selected | 13,205 | 9235 | |
Initial particle images (no.) | 8,542,808 | 3,909,861 | |
Final particle images (no.) | 200,134 | 201,630 | 163,391 |
Symmetry imposed | C1 | C1 | C1 |
FSC threshold | 0.143 | 0.143 | 0.143 |
Final map resolution (Å) | 2.84 | 2.88 | 3.00 |
Atomic model | |||
Number of protein residues | 1142 | 1121 | 1149 |
Validation | |||
Favored (%) | 97.35 | 97.20 | 98.23 |
Allowed (%) | 2.65 | 2.80 | 1.77 |
Outliers (%) | 0.00 | 0.00 | 0.00 |
Rotamer outliers (%) | 0.11 | 0.69 | 0.56 |
r.m.s.d Bond lengths (Å) | 0.003 | 0.004 | 0.004 |
r.m.s.d Bond angles (°) | 0.596 | 0.640 | 0.647 |
Clashscore | 8.04 | 8.40 | 5.99 |
Map CC | 0.87 | 0.88 | 0.87 |
Deposition ID | |||
PDB | |||
EMDB | |||
