Fig. 1: Response of the Mtb metabolome and lipidome to glpQ1 deletion.
a Volcano plot showing the metabolome of Mtb plotted as the fold change in the mean abundance of each feature in ΔglpQ1/WT, showing negative ion-mode. Features in orange and red are statistically significantly enriched in the ΔglpQ1 mutant and represent unidentified and identified features respectively, features in blue are statistically significantly depleted in the ΔglpQ1 mutant. Means were calculated across replicate cultures: ΔglpQ1 = 6 replicates, WT = 8 replicates. Representative of two independent experiments. P values are from two-sided Student’s t-test, and are adjusted for multiple comparisons setting a false discovery rate of 5%. b Extracted ion chromatograms for each of the four lipid-heads of Mtb, shown beneath the chemical structure of the neutral molecule. Note that the chromatogram for each replicate culture within each strain are overlain. P values shown in grey refer to the level of significance of the changes in mean intensity for the ion between the strains indicated. c Plots of the abundance of the five major phospholipid classes of Mtb in each strain, as found by lipidomic analysis. The bars represent the mean abundance of the lipid class in each strain, drawn as relative to the level in the WT (mean abundance in the WT set to 1). For each lipid class, the 5 most abundant species of lipid were summed. Bar height represents the mean summed abundance across replicate cultures for that strain, the error bars show the standard deviation. Individual points represent the summed abundance for each individual replicate culture of that strain. P values are the 2 tail t-test comparing the abundance in the ΔglpQ1 mutant versus the WT. The plots display two independent experiments side by side (Experiment 1: WT = 5 replicates, ΔglpQ1 = 6 replicates, ΔglpQ1::glpQ1_Pimyc = 4 replicates. Experiment 2: WT = 5 replicates, except for PIM2 = 4 replicates, ΔglpQ1 = 5 replicates, ΔglpQ1::glpQ1_Pimyc = 5 replicates). Data shown is from positive ion-mode MS, except for PIM2 which is negative ion-mode. Source data are provided as a Source Data file.
