Fig. 7: Unconventional CD4+ T cells in children with T1D. | Nature Communications

Fig. 7: Unconventional CD4+ T cells in children with T1D.

From: Imbalance of stem-like and effector T cell states in children with early type 1 diabetes across conventional and regulatory subsets

Fig. 7

A UMAP of CD4⁺ T cells showing merged Louvain clusters used for subset analysis (n = 79,876 cells from 43 donors). B Same UMAP annotated with a published dataset of TR3-56 (CD3⁺CD56⁻), NK cells, CD3⁺CD56⁻, and CD8⁺ cells (GSE106082) using SingleR. Cells annotated as TR3-56 are highlighted. C Percentage of TR3-56–annotated cells in each cluster. D Violin plots of TR3-56 annotation scores (similarity to TR3-56 reference) by cluster; bars = medians. E Violin plots of the percentage of cells per cluster with non-zero expression of selected genes (87 samples); bars = medians. F Reclustering of CD4⁺ Temra cells from (A). In the resulting UMAP, there are n = 815 cells from 43 donors. G Heatmap of selected marker genes for clusters in (F), colored by row-scaled z-scores of average expression. H Percentage of cells in subclusters from (F) among total CD4⁺ Temra cells in healthy (n = 13), T1D T0 (n = 30), and T1D T1 (n = 29) donors. P-values by two-tailed Mann–Whitney test (healthy vs. T1D T0) or two-tailed paired Mann–Whitney test (T1D T0 vs. T1); bars = medians. AgExp – Antigen Experienced T cells, T1D – Type 1 Diabetes Mellitus, T0 – timepoint at diagnosis, T1 – timepoint one year after diagnosis.

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