Fig. 2: Associations of plasma proteins with incident Crohn’s disease.
From: Plasma proteomic profiles identify biomarkers predicting Crohn’s disease up to 16 years before onset
a Volcano plots showing the HR (x axis) and −log10(P value) (y axis) for the global associations of 2736 proteins with incident Crohn’s disease in the training set. All results for both Cox proportional hazard regression models 1 and 2 are shown here. Model 1 was adjusted for age, sex, and ethnicity. Model 2 was additionally adjusted for BMI, Townsend deprivation index, smoking, alcohol intake frequency, physical activity, dietary habits, anxiety, depression, and medication history of antibiotics and NSAIDs. P values were calculated under two-sided Wald tests and no multiple comparisons were applied. Proteins above the horizontal dotted black line were significantly associated with incident Crohn’s disease after Bonferroni corrections (P < 0.05) taking into account the number of proteins tested (n = 2736). b Enrichment for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Significant proteins after Bonferroni correction derived from Cox proportional hazard regressions in model 1 or model 2 were fed into the DAVID website (https://david-d.ncifcrf.gov) for enrichment analysis. P values were calculated under two-sided tests and statistical significance was defined as a false discovery rate corrected P < 0.05 (dotted horizontal line). The number above each bar is the number of observed proteins in each pathway. Detailed results are shown in Supplementary Data 2. Abbreviations BP biological process, CC cellular component, MF molecular function, TNF tumour necrosis factor.
