Table 3 List of nematode and bacterial strains used in this study

From: A noncanonical polyamine from bacteria antagonizes host mitochondrial function

Strain

Description

Reference

Nematode strains

  

N2

wild type C. elegans

CGC

DR1572

C. elegans daf-2(e1368) III

CGC

NOB159

C. elegans catp-5(bur50) G855N

this study

NOB160

C. elegans catp-5(bur51) G400E

this study

NOB161

C. elegans catp-5(bur52) W59*

this study

NOB162

C. elegans catp-5(bur53) G466N

this study

NOB163

C. elegans catp-5(bur54) G1101R

this study

NOB164

C. elegans catp-5(bur55) intron variant

this study

NOB165

C. elegans catp-5(bur56) G441R

this study

NOB166

C. elegans catp-5(bur57) S553R

this study

NOB167

C. elegans catp-5(bur58) T705M

this study

NOB168

C. elegans catp-5(bur59) intron variant

this study

NOB273

C. elegans dhps-1(ve590); hsp-6p::GFP

this study

IG274

C. elegans frIs7 [nlp-29p::GFP + col-12p::DsRed] IV

CGC

JIN810

C. elegans agIs26 [clec-60p::GFP + myo-2p::mCherry]

CGC

LD1171

C. elegans ldIs3 [gcs-1p::GFP + rol-6(su1006)]

CGC

SJ4058

C. elegans zcIs9 [hsp-60::GFP + lin-15(+)]

CGC

WE5172

C. elegans ajIs1 [rCesC05A9.1::GFP + dpy-5( + )] X

CGC

CL2070

C. elegans dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]

CGC

CL2166

C. elegans dvIs19 [(pAF15)gst-4p::GFP::NLS] III

CGC

SJ4005

C. elegans zcIs4 [hsp-4::GFP] V

CGC

AU133

C. elegans agIs17 [myo-2p::mCherry + irg-1p::GFP] IV

CGC

CF1553

C. elegans muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]

CGC

CY573

C. elegans bvIs5 [cyp-35B1p::GFP + gcy-7p::GFP]

CGC

GL347

C. elegans zcIs13 [hsp-6p::GFP + lin-15( + )] V

CGC

PS312

P. pristioncus wild isolate

CGC

RG3090

C. elegans dhps-1(ve590)

CGC

B. subtilis strains

–

–

BGSCID:1A1

B. subtilis 168 trpC2

35

NOBb369

B. subtilis 168 ΔspeB

this study

NOBb475

B. subtilis 168 ΔspeB thrC::PspeEB-speB (pNOB47)

this study

NOBb501

B. subtilis 168 ΔspeEB thrC::PspeEB-speE (pNOB48)

this study

NOBb503

B. subtilis 168 ΔspeB speA::kanR thrC::PspeA-speA (pNOB49)

this study

NOBb513

B. subtilis 168 thrC::pDG1664

this study

NOBb514

B. subtilis 168 ΔspeB thrC::pDG1664

this study

NOBb515

B. subtilis 168 ΔspeEB thrC::pDG1664

this study

NOBb516

B. subtilis 168 speA::kanR thrC::pDG1664

this study

NOBb517

B. subtilis 168 speE::kanR thrC::pDG1664

this study

NOBb518

B. subtilis 168 ΔspeB speA::kanR thrC::pDG1664

this study

E. coli strains

–

–

OP50

ura-, strR, rnc-, (delta)attB::FRT-lacI-lacUV5p-T7)

79

K12(BW25113)

F- DE(araD-araB)567 lacZ4787(del)::rrnB-3 LAM- rph-1 DE(rhaD-rhaB)568 hsdR514

63

NOBb806

K12 ΔspeB

this study

NOBb914

K12 ΔspeB speE::kanR

this study

NOBb866

K12 ΔspeB ΔspeF speC::kanR

this study

NOBb916

K12 ΔspeB ΔspeF ΔspeC speE::kanR

this study