Table 4 List of plasmids used in this study
From: A noncanonical polyamine from bacteria antagonizes host mitochondrial function
Plasmid | Relevant features | Parent vector | Restriction enzymes to digest parent vector | PCR primers | PCR template | Reference |
|---|---|---|---|---|---|---|
pDG1664 | thrC, amp, erm, spec | N/A | N/A | N/A | N/A | |
pMAD | amp, erm, ori-pE194ts | N/A | N/A | N/A | N/A | |
pNOB41 | pMAD ΔspeEB | pMAD | BglII, EcoRI | KN429-KN432 | B. subtilis 168 | this study |
pNOB47 | thrC::PspeEB-speB amp erm spec | pDG1664 | BamHI, EcoRI | KN475-KN478 | B. subtilis 168 | this study |
pNOB48 | thrC::PspeEB-speE amp erm spec | pDG1664 | BamHI, EcoRI | KN492, KN493 | B. subtilis 168 | this study |
pNOB49 | thrC::PspeA-speA amp erm spec | pDG1664 | BamHI, EcoRI | KN490, KN491 | B. subtilis 168 | this study |
