Fig. 2: MET-2 regulates monoallelic expression when coding sequence and locus are changed.

For each row, a–d, left panel “torso” images of control and experimental worms with a 10 micrometer white scale bar inset in the bottom left of each control animal; middle panel scatter plots of control and experimental group intestine cells plotted by allele expression values from all three independent experiments; right panel boxplots of intrinsic noise values from cells from control and experimental worms. Top of boxplot is 75th percentile, bottom of box is 25th percentile, line is median, top and bottom error bars are 90th and 10th percentile, respectively, and dots are 95th and 5th percentile. a Intestine cells from met-2(null) animals had a significantly higher median intrinsic noise (0.187 compared to 0.00232 for control animals); P < 0.001, Mann–Whitney Rank Sum Test, two-sided, N = 209 cells for control, N = 208 cells for met-2(null), three independent experiments. b Worms have reporter alleles inserted at a Chr. V locus. Intestine cells from met-2(RNAi) animals had a significantly higher median intrinsic noise (0.180 vs control 0.0196), P < 0.001, Mann–Whitney Rank Sum Test, two-sided, N = 207 cells for control, N = 206 cells for met-2(RNAi), three independent experiments. c Worms have full length hsp-90 reporter alleles inserted at the same Chr. II locus as in (a). Intestine cells from met-2(RNAi) animals had a significantly higher median intrinsic noise (0.100 vs 0.00163 for control animals), P < 0.001, Mann–Whitney Rank Sum Test, two-sided, N = 196 cells for control, N = 210 cells for met-2(null), three independent experiments.