Fig. 3: MET-2 negatively regulates monoallelic expression in a promoter/gene-specific fashion.

For each row, a–d, left panel “torso” images of control and experimental worms with a 10 micrometer white scale bar inset in the bottom left of each control animal; middle panel scatter plots of control and experimental group intestine cells plotted by allele expression values from all three independent experiments; right panel boxplots of intrinsic noise values from cells from control and experimental worms. Top of boxplot is 75th percentile, bottom of box is 25th percentile, line is median, top and bottom error bars are 90th and 10th percentile, respectively, and dots are 95th and 5th percentile. a no significant difference in allele bias for vit-2 reporter allele expression between met-2(null) animals and controls (0.000809 vs 0.000968 control), P = 0.266, Mann–Whitney Rank Sum Test, two-sided, N = 210 cells for control, N = 209 cells for met-2(null), three independent experiments. b no significant difference in allele bias for hsp-16.2 reporter allele expression between met-2(RNAi) animals and controls (0.00311 vs 0.0297 control), P = 0.595, Mann–Whitney Rank Sum Test, two-sided, N = 210 cells for each group, three independent experiments. c no significant difference in allele bias for fluorescently tagged idh-1 reporter alleles between met-2(RNAi) animals and controls (0.00337 vs 0.00356 control), P = 0.730, Mann–Whitney Rank Sum Test, two-sided, N = 208 cells for control, N = 209 cells for met-2(RNAi), three independent experiments. d Intestine cells from eef-1A.1(null) animals had a significantly higher median intrinsic noise than controls (0.00944 compared to 0.00254 for controls); P < 0.001, Mann–Whitney Rank Sum Test, N = 180 cells for each group, three independent experiments. eef-1A.1 reporter alleles were labeled with mTagBFP2 and mNeptune.