Fig. 5: Cysteine to alanine mutation in the SET domain of SET-25 or MET-2 causes loss of monoallelic expression regulation. | Nature Communications

Fig. 5: Cysteine to alanine mutation in the SET domain of SET-25 or MET-2 causes loss of monoallelic expression regulation.

From: Maternal histone methyltransferases antagonistically regulate autosomal random monoallelic expression (aRMAE) in C. elegans

Fig. 5

For each row, (d–f), left panel “torso” images of control and experimental worms with a 10 micrometer white scale bar inset in the bottom left of each control animal; middle panel scatter plots of control and experimental group intestine cells plotted by allele expression values from all three independent experiments; right panel) boxplots of intrinsic noise values from cells from control and experimental worms. Top of boxplot is 75th percentile, bottom of box is 25th percentile, line is median, top and bottom error bars are 90th and 10th percentile, respectively, and dots are 95th and 5th percentile. a Graphical alignment of human SETDB1 and SUV39 with worm homologs. Predicted domains are highlighted by different colored boxes. b Text alignment of MET-2, SETDB1, SET-25, and SUV39 with conserved cysteine residue in red. c Text alignment of the SET domain of met-2(cat) and set-25(cat) mutants. These mutations change a conserved cysteine to alanine. d met-2(cat) phenocopies the null mutant (Fig. 2a), with significantly higher intrinsic noise than control animals; 0.134 for met-2(cat) vs. 0.00459 in controls, P < 0.05, Kruskal–Wallis One Way Analysis of Variance on Ranks followed by Dunn’s Method for Multiple Comparison, N = 209 cells for control, N = 210 cells for met-2(cat), three independent experiments. e set-25(cat) phenocopies the set-25 RNAi (Fig. 4f), with significantly lower intrinsic noise than controls; 0.00114 in set-25(cat) vs 0.00459 for control, P < 0.05, Kruskal–Wallis One Way Analysis of Variance on Ranks followed by Dunn’s Method for Multiple Comparison, N = 209 cells for control, N = 210 cells for set-25(cat), three independent experiments. f met-2(cat);set-25(cat) double SET- domain mutant animals have significantly lower intrinsic noise than met-2(cat) animals and hsp-90 control animals; 0.00140 for met-2(cat);set-25(cat) vs. 0.00459 for control or 0.134 for met-2(cat), P < 0.05 for both comparisons, Kruskal–Wallis One Way Analysis of Variance on Ranks followed by Dunn’s Method for Multiple Comparison, N = 209 cells for control, N = 210 cells for met-2(cat), 210 cells for met-2(cat);set-25(cat), three independent experiments.

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