Table 1 Targeted genetic screen for negative regulators of monoallelic expression

From: Maternal histone methyltransferases antagonistically regulate autosomal random monoallelic expression (aRMAE) in C. elegans

Gene

Predicted or confirmed function

Coefficient of determination (R2) for alleles

Empty vector

Normal wild-type functions under ad libitum laboratory conditions

0.928 (SD = 0.04)

set-1

Predicted HMT

RNAi lethal

set-2

H3K4 HMT, SETD1a

0.9547

set-3

Predicted HMT, SMYD4

0.9355

set-4

Predicted HMT, H4K20 specific, KMT5C

0.8564

set-5

Predicted HMT, KMT2E/SETD5

0.9167

set-6

Predicted HMT, H3K9?

0.845

set-7

ATP binding, TTLL12

0.9333

set-8

SET domain

0.8237

set-9

SET domain, set-26 paralog, SETD5, H3K4me3 reader

0.5827

set-10

Predicted HMT, distant SYMD1/2

0.7147

set-11

Predicted HMT, p53 binding, EHMT1/2/SUV39H1/H2

0.8747

set-12

Predicted HMT, H3K36 specific, NSD1/2

0.9089

set-14

Predicted HMT, SMYD2

0.7095

set-15

SET domain

0.8311

set-16

H3K4 specific HMT, KMT2D

0.9028

set-18

H3K36 specific HMT, SMYD3

0.8217

set-19

SET domain

0.8035

set-20

SET domain

0.9422

set-21

Predicted H3K9 HMT?

0.9691

set-22

SET domain

0.9656

set-24

SET domain

0.9451

set-25

H3K9-specific HMT

0.963

set-27

Predicted H4K4 and H3K36 HMT, SETD3

0.9312

set-28

SET domain

0.9122

set-30

Predicted H3K4 specific HMT, SMYD1/2/3

0.9683

set-31

SET domain

0.9083

set-32

H3K23me3 HMT

0.9444

hpl-2

Human HP1, condensed chromatin

0.9804

met-2

H3K9me1/2 specific HMT, SETDB1

0.2753

mes-2

H3K27 specific HMT, EZH1/2, polycomb/PRC2, X-inact

0.98

mes-4

H3K36 specific HMT, silences repetitive arrays

0.9364

utx-1

H3K27 specific demethylase, KDM6A/B UTX/UTY

0.9206

jmjd1.2

H3K9me2 demethylase

0.7343

jmjd-2

H3K36me3 demethylase

0.9722

jhdm-1

H3K36me3 demethylase

0.9685

spr-5

H3K4 demethylase, KDM1A

0.887

wdr-5

H3K4 HMT, MLL/ set/COMPASS, WDR5

0.9233

lsd-1

H3K4 demethylase, KDM1A

0.9606

mrg-1

H3K4me/H4K16ac transcriptional silencing

0.9453

prg-1

Piwi RNA-mediated gene silencing, PIWIL1

0.8671

hrde-1

RNAi effector, exo-RNAi targets, RISC complex

0.9012

ergo-1

26 G RNAi pathway, repression of repetitive sequences

0.9539

nrde-2

Recruited to nrde-3 complexes, exo-RNAi RISC complex

0.9788

nrde-3

RNAi effector, exo-RNAi targets in soma

0.9452

nrde-4

Recruited to nrde-3 complexes, exo-RNAi RISC complex

0.7605

sago-1

AGO, siRNA amplification exo RNAi

0.9195

sago-2

AGO, siRNA amplification exo RNAi

0.8247

dcr-1

Required for RNAi, small RNA processing

0.9196

morc-1

Endo-siRNA, HUSH complex

0.8926

nmad-1

DNA methylase, ALKBH4

0.9514

damt-1

DNA methylase (adenine), METTL4

0.8961

  1. RNAi Screen for regulators of allele bias. Genes screened, their function, and allele bias score (R2, coefficient of determination for all cells in all worms, n = 70 cells from 10 worms). Control empty vector RNAi was conducted in triplicate, with mean and +/− SDEV shown.
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