Fig. 3: Analysis of itaconated secreted proteins identified by PBSP. | Nature Communications

Fig. 3: Analysis of itaconated secreted proteins identified by PBSP.

From: Spatiotemporal profiling of modification-specific proteome secretion uncovers an itaconation-activated tyrosine kinase

Fig. 3

a Comparison of itaconated proteins identified by PBSP with those previously identified through intracellular ITalk labeling and S-glycosylation-based competitive cysteine profiling. b Proportion of known secretory proteins among the itaconated secreted proteins identified by PBSP. c KEGG pathway analysis of the itaconated secreted proteins identified by PBSP with one-sided p-values from Fisher’s Exact test. d KEGG pathway analysis of the newly identified itaconated proteins with one-sided p-values from Fisher’s Exact test. e Enrichment ratios of representative itaconated secreted proteins involved in COVID-19, including the RNA sensor RIG-I, MAPK1, and MAPK3. p-values: 0.00571 (MAPK3), 0.00448 (MAPK1), 0.01017 (RIG-I). f Enrichment ratios of representative itaconated secreted enzymes in the lysosome. p-values: 0.00277 (ATP6V1H), 0.01711 (ACP5), 0.04596 (PLA2G15), 0.00152 (CTSC), 0.01452 (CTSL), 0.02720 (CTSS). g Enrichment ratios of representative itaconated secreted proteins involved in ubiquitination pathway. p-values: 0.03241 (USP5), 0.01159 (USP39), 0.01457 (USP9X), 0.01744 (USP19), 0.02384 (USP14). In (e–g), the error bars show mean ± SD from three biological replicates. *p < 0.05, **p < 0.01 (two-sided Student’s t-test).

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