Fig. 6: Identification of succinated secreted proteins via PBSP. | Nature Communications

Fig. 6: Identification of succinated secreted proteins via PBSP.

From: Spatiotemporal profiling of modification-specific proteome secretion uncovers an itaconation-activated tyrosine kinase

Fig. 6

a Workflow for mapping succinated secreted proteins with PBSP. Created in BioRender. Chu, l. (2025) https://BioRender.com/yx6pwe1. b Volcano plot showing the comparative enrichment of proteins between Fumarate-alkyne labeling group and control group by PBSP. Succinated secreted proteins are highlighted in red. p-values were determined by two-sided Student t-tests. c Proportion of known secretory proteins among the succinated secreted proteins identified by PBSP. d KEGG pathway analysis of the succinated secreted proteins identified by PBSP with one-sided p-values from Fisher’s Exact test. e Overlap of succinated and itaconated secreted proteins identified by PBSP. f Volcano plot comparing protein enrichment under control vs. exosome-inhibited conditions (GW4869 treatment). Exosome-dependent succinated secreted proteins are highlighted in red. p-values were determined by two-sided Student t-tests. g Proportion of known exosome proteins within the exosome-dependent succinated secreted proteins. h KEGG analysis of the exosome-dependent succinated secreted proteins with one-sided p-values from Fisher’s Exact test.

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