Fig. 2: Hypusination inhibition causes significant loss of newly synthesized proteins from 6 h. | Nature Communications

Fig. 2: Hypusination inhibition causes significant loss of newly synthesized proteins from 6 h.

From: Inhibiting translation elongation by reducing eIF5A activity induces feedback inhibition of initiation, limiting tumour cell proliferation

Fig. 2: Hypusination inhibition causes significant loss of newly synthesized proteins from 6 h.The alternative text for this image may have been generated using AI.

a The proportion of newly synthesized proteins in GC7 and control treated A549 cells determined by dynamic SILAC. Three independent biological replates were used for each condition and each time point. b Volcano plots showing proteins significantly changing after GC7 treatment at each time point. Differential abundance of newly synthesized proteins was analyzed using DEqMS with the log₂-transformed ratio of heavy to light intensities. Tests were two-sided and p-values were adjusted for multiple testing using the Benjamini–Hochberg False Discovery Rate (FDR) procedure. c Gene Ontology (GO) terms significantly over-represented among proteins showing a significant loss of synthesis at 6 h. Over-representation analysis was performed using goseq and the reported one-sided p-values were adjusted for multiple testing using the FDR procedure. BP Biological Process; CC Cellular Compartment; MF Molecular Function. d Empirical cumulative density plots for the difference in newly synthesized protein between GC7 and control for proteins with known mitochondrial subcellular localizations.

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