Fig. 5: Single molecule analysis reveals telomere DNA dynamics within non-productive telomerase complexes after 6-thio-dG addition. | Nature Communications

Fig. 5: Single molecule analysis reveals telomere DNA dynamics within non-productive telomerase complexes after 6-thio-dG addition.

From: Therapeutic 6-thio-deoxyguanosine inhibits telomere elongation in cancer cells by inducing a non-productive stalled telomerase complex

Fig. 5

a Representative traces of stalled single telomerase-DNA complexes (top), in the presence of dNTPs (second panel), with dATP, dTTP and 6-thio-dGTP (6dGTP) (third panel), and with dATP, dTTP, dGTP, and a 10-fold excess of 6-thio-dGTP (bottom panel). Data were collected 15 minutes after the addition of dNTPs. Raw data collected at 8.3 Hz framerate are shown in black, and a one-second moving average is overlaid in red. Black arrows indicate irreversible photobleaching of the FRET dyes. b Heat map analysis of the time-dependent FRET signal of ~ 100 individual telomerase-DNA complexes in each experimental condition. Small black arrows in the top panel indicate the FRET state of the stalled complex (upper arrow) and the FRET when the dyes have photobleached, also indicated by the signal at 0 FRET. In the presence of dNTPs (second panel), dATP, dTTP, 6-thio-dGTP (third panel) or dATP, dTTP, dGTP and 10-fold excess 6-thio-dGTP (bottom panel), broadening of the FRET distribution (solid black lines) indicates DNA dynamics within the telomerase-DNA complexes. Source data are provided as a Source Data file.

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