Fig. 3: The repair of single-strand discontinuities requires recombination.
From: Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading
A Spot-titre analysis of recombinational repair mutants in strains engineered to express (+ xylose) nCas9. sgRNAs were targeted to a locus at -90°. The parental nicking strain control is shown above. B MFA using whole genome sequencing of recombinational repair mutants in the presence or absence of the weak nCas9 system. The frequency of sequencing reads was plotted against genome position. C Diagram illustrating proposed molecular events required for recombinational repair of a seDSB created after a replication fork encounters a single-strand discontinuity in the leading strand template. Source data are provided as a Source Data file.
