Fig. 7: RecG and RecU are recruited to sites of recombinational repair.
From: Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading
A ChIP-qPCR analyses of FLAG-RecG in strains engineered to express nCas9. Top panel shows the genetic system employed in this experiment. The sgRNAs targeted nCas9 to a locus located at -90° and protein enrichment was normalised to the control locus at +90°. Error bars indicate the standard error of the mean and circles overlaid on bars correspond to three biological replicates. B Fluorescence microscopy images of ΔrecU mutant strains engineered to express nCas9 (sgRNAs targeted to -90°). Grey scale images correspond to phase contrast. Blue signal corresponds to DAPI staining (DNA) and red signal corresponds to Nile red staining (membrane) in composite fluorescence images. Cas9 proteins were induced (+ xylose) during exponential growth phase for 90 minutes before cells were collected for imaging. Scale bar indicates 5 µm. C ChIP-qPCR analyses of RecU-FLAG in a strain engineered to express nCas9. Top panel shows the genetic system employed in this experiment. The sgRNA targeted nCas9 to a locus located at -90° and protein enrichment was normalised to the control locus at +90°. Error bars indicate the standard error of the mean and circles overlaid on bars correspond to three biological replicates. Source data are provided as a Source Data file.
