Fig. 5: Lowering of F-actin in oenoncytes can rescue the sequestration of lipoproteins in OEts>Desat1RNAi animals.
a Representative immunofluorescence images of phalloidin (red) in central or cortical sections of oenocytes. Actin depolymerization via knocking down Limk1 or overexpressing Tsr reduces cortical actin accumulation. n = 4, scale bars: 20 μm. b Representative immunofluorescence images of apoLpp in central or cortical sections of oenocytes. Dashed lines mark the oenocytes, n = 3, scale bars: 20 μm. c Western blot analysis of apolpp in the hemolymph. OEts>Desat1RNAi exhibit a reduced level of apolpp in the hemolymph compared with OEts>ControlRNAi, which can be rescued by actin depolymerization. Only one band was seen after Ponceau staining, and this was used for normalization. d Quantification of apolpp in hemolymph, n = 6. Statistical tests: one-way ANOVA. *, P < 0.05; **, P < 0.01; ****, P < 0.0001. Data are presented as mean values ± SD. e TAG levels at different days of starvation in whole fly sample measured by TAG kit, n = 5. Statistical tests: Two-way ANOVA. **, P < 0.01; ***, P < 0.001. Data are presented as mean values ± SD. f starvation assay at 29°C. n = 150, P value was calculated using Log-rank (Mantel-Cox) test. P < 1×10-15(ControlRNAi vs. Desat1RNAi). ****, P < 0.0001. Data are presented as percent survival values ± SEM. Source data for plots and exact p-value are provided as a source data file.
