Fig. 5: Skeletal muscle proteome remodelling differs following MICT and SIT.
A Schematic of the exercise training sessions completed throughout the 8 weeks. In black, the experimental exercise session where muscle biopsies were collected. B Markers of mitochondrial content from whole muscle, including total oxidative phosphorylation (OXPHOS) protein content (left graph; MICT, n = 11; SIT, n = 12; a.u. arbitrary units); mitochondrial volume density (MitoVD; middle graph; MICT, n = 10; SIT, n = 12), and citrate synthase activity (CS; right graph; MICT, n = 11; SIT, n = 12). C Mitochondrial respiratory function was measured in permeabilized fibers. Left graph: respiration from electron-transferring flavoprotein (ETFp; in the presence of 0.2 mM octanyolcarnitine, 2 mM malate, 3 mM MgCl2 and 5 mM ADP); middle graph: respiration after addition of complex I- and complex II-linked substrates (ETF+ CI+ CIIP; in the presence of 5 mM pyruvate, 10 mM succinate, 3 mM MgCl2 and 5 mM ADP); right graph: electron transport system (ETS) maximal capacity obtained following titration with the uncoupler FCCP [0.7–1.5 mM]) following MICT (n = 8) and SIT (n = 10). See ‘Material and methods’ for details. D Representative blots of proteins used to calculate total OXPHOS abundance. E Representative micrographs from PRE and POST exercise training for each group. Scale bar = 1 µm. F Proteomic analysis workflow. G Principal component analysis (PCA) from the proteome analysis across both groups (MICT, n = 11; SIT, n = 12). H Venn diagram showing that only a fraction of the differentially expressed proteins are shared across groups despite a largely similar PCA plot. I Protein enrichment analysis across groups with using Reactome pathways. * denotes main effect at p < 0.05. Data are expressed as mean. For B and C data were analyzed using pre-planned one-way ANOVA. Source data are provided as a Source Data file. Created in BioRender. Bishop, D. (2025) https://BioRender.com/ub4yev6.
