Fig. 8: Macroscopic currents from subunit-omitted muscle AChRs.
From: Disentangling the mechanistic role of loop-C capping in Cys-loop receptor activation
a, b Inward currents recorded from the indicated constructs in the whole-cell patch-clamp configuration in response to 20-ms (a) or 1.8-s (b) exposures to 100-μM ACh. For the wild-type mouse-muscle AChR (adult type), responses are shown as the mean (black solid line) ± 1 SD (gray error bars) of normalized responses recorded from different whole-cell experiments. Averaged wild-type data in (a) correspond to a total of 95 responses recorded from 12 different cells, and those in (b) correspond to a total of 10 individual responses recorded from 10 different cells. Each normalized trace shown for the subunit-omitted mutants corresponds to a single response. The pipette potential was –60 mV. Black dashed lines denote the zero-current baseline. Source data are provided as a Source Data file.
