Fig. 2: Ablation of Raptor in ILC2s ameliorates allergic asthma.

a–d Frequency and total number of IL-5⁺ ILC2s (a,b) and IL-13⁺ ILC2s (c, d) in the lung of R5/+ and R5/+ Rptor^f/f (KO) mice (n = 5). Cells were gated on CD45⁺lin⁻CD25⁺ST2⁺ live cells. e–p R5/+ and R5/+ Rptor^f/f (KO) mice were challenged with papain intranasally (i.n.). Lung tissues were collected and analyzed on day 6 (n = 6). e, f Frequency and absolute counts of lung ILC2s. g, h Frequency of Ki67⁺ ILC2s (n = 5). i–l Frequency and absolute counts of IL-5⁺ ILC2s (i, j) and IL-13⁺ ILC2s (k, l). m The levels of IL-5 and IL-13 in the BALF were measured by ELISA. n, o Frequency and cell number of Eosinophils (CD45⁺CD11b⁺Gr-1⁻SiglecF⁺CD11c⁻) in bronchoalveolar lavage fluid (BALF) (n) and lung (o). p Paraffin-embedded lung sections were stained with Alcian Blue–Periodic Acid–Schiff (AB-PAS) and AB-PAS⁺ (positive) area was quantified by ImageJ. Scale bars, 50μm. Each symbol represents an individual mouse (a–p). Data were pooled from two independent experiments, shown as mean ± SEM. Statistical significance was determined by a two-tailed unpaired Student’s t test in (a–p). p-values are shown on the graphs. Source data are provided as a Source Data file.