Fig. 3: mTORC1 regulates lung ILC2 function in a cell-intrinsic manner.

a–e Nmur1^iCre-TdT and Nmur1^iCre-TdT Rptor^f/f mice were challenged with papain. Lung tissues were collected and analyzed on day 6 (n = 6). a–d Frequency and absolute numbers of total ILC2s (a), IL-5⁺ ILC2s (b), IL-13⁺ ILC2s (c), and eosinophils (d) in the lung. e Representative Alcian Blue–Periodic Acid–Schiff (AB-PAS) staining of lung sections and histological quantification of AB-PAS⁺ (positive) area by ImageJ. Scale bars, 50 μm. f–i Lung ILC2s sorted from R5/+ (Control) or R5/+ Rptor^f/f (KO) mice were transferred intravenously into Rag2^–/–Il2rg^–/– mice, which were challenged with papain subsequently. Lung tissues were collected and analyzed on day 7 (n = 4). f Frequency and absolute counts of ILC2s from recipients after ILC2 transfer. Cells were gated on CD45⁺lin⁻ live cells. g, h Frequency and cell number of IL-5⁺ ILC2s, IL-13⁺ ILC2s (g), and eosinophils (live CD45⁺GR1^-CD11c⁻SiglecF⁺) (h) in the lungs. i Representative AB-PAS staining of lung sections and histological quantification of AB-PAS⁺ (positive) area by ImageJ. Scale bars, 50 μm. Each symbol represents an individual mouse (a–i). Data were pooled from two independent experiments (a–e), and data are representative of two (f–i) independent experiments shown as mean ± SEM. Statistical significance was tested by a two-tailed unpaired Student’s t test in (a–i). p-values are shown on the graphs, and p-values < 0.0001 are reported as such. Source data are provided as a Source Data file.