Fig. 1: Mitochondrial morphometrics in mouse neocortical microglia.

a In vivo image of mitochondrial labeling (green) in microglia (magenta) in the upper layers of the mouse somatosensory cortex. The images on the bottom show mitochondrial partitioning in 1 microglia (left image), representative soma from three separate microglia (middle images) and the two processes cropped from the first microglia as indicated and numbered. b The mitochondrial to cytoplasm volume fraction for a subset of microglia that were segmented in 3D. Values are shown for the total cell, the soma, and the processes. Each dot in the graph represents 1 cell. c Differences in the average mitochondrial network volume and number of mitochondrial networks between the soma and processes. d 3D segmentation of a single microglia and its mitochondria and morphological analysis via Sholl reveals increased process complexity (magenta line) at 20 microns from the cell center but linear mitochondrial signals (green line) radiating from the center of the cell. e The mitochondrial occupancy along the length of a microglial primary process in 10% bins starting from the center of the cell (0-10%) and going to the tip of the process (91–100%) (n = 117 primary processes from 14 cells from 6 mice, error bars indicate s.e.m.) f Mitochondrial circularity and aspect ratio in the soma and processes. (for b–f, n = 14 cells from 6 mice, paired two-tailed t-test, the line is at the mean ± s.e.m for error bars or error bands.) For (b, c, f), see Supplementary Figs. 3a–c and 5a–c for analyses by animal and of sex differences, respectively.