Fig. 5: Microglial microtubule networks mimic the heterogeneous partitioning of mitochondria. | Nature Communications

Fig. 5: Microglial microtubule networks mimic the heterogeneous partitioning of mitochondria.

From: Mitochondria are absent from microglial processes performing surveillance, chemotaxis, and phagocytic engulfment

Fig. 5

a Strategy for Cre recombinase dependent conditional labeling and imaging of microglial cytoplasm and their microtubules (MTs) and mitochondria using floxed tdTomato, eGFP, and mKate2 fluorescent reporters, respectively. b Microglial cytoplasm (red), MTs (cyan), and mitochondria (yellow) were visualized in vivo using cranial window surgeries and intravital 2-photon imaging. c Representative microglia showing processes containing both MTs and mitochondria (magenta arrows) and processes lacking both (white arrows). d Comparing area fraction of MTs and mitochondria in single microglia (n = 19 microglia from 5 mice; paired two-tailed t-test). e Binary analysis showing the percentage of processes with and without mitochondria that contain MTs (n = 24 processes with mitochondria and 24 processes without mitochondria from 12 microglia and 6 mice; two-sided Fisher’s exact test). For (d, e) see Supplementary Figs. 3e and 5e, f for analyses by animal and of sex differences, respectively.

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