Fig. 1: Schematic representation of common plant transformation techniques.
From: The evolving landscape of precise DNA insertion in plants
Agrobacterium-mediated transformation involves the introduction of transfer DNA (T-DNA) into plant cells, leading to stable integration. Particle bombardment, on the other hand, propels DNA-coated gold/tungsten particles into plant cells, resulting in random insertion of DNA into the genome. Polyethylene glycol (PEG)-mediated protoplast transfection introduces purified DNA into isolated protoplasts, which can then regenerate into whole plants. These techniques facilitate the creation of transgenic plants from single transformed cells. pTi: tumor-inducing plasmid. Created in BioRender. https://BioRender.com/guc179v.
