Fig. 7: Activation of the DbfQRS pathway results in a competitive disadvantage in vitro and in vivo. | Nature Communications

Fig. 7: Activation of the DbfQRS pathway results in a competitive disadvantage in vitro and in vivo.

From: A small periplasmic protein governs broad physiological adaptations in Vibrio cholerae via regulation of the DbfRS two-component system

Fig. 7

a In vitro competition assay. WT (lacZ⁻) V. cholerae were competed against the indicated strains at a 1:1 ratio in LB medium for 24 h. Data are displayed as medians with interquartile ranges. Points represent individual replicates of N = 3 biological replicates. Statistical analyses were performed using unpaired, two-sided t-tests with a 95% confidence interval. P = 0.0021, 0.0016, 0.0977, 0.3696, and 0.0027 for the indicated pairs. b In vivo competitive colonization assay. WT (lacZ⁻) was competed against the indicated strains at a 1:1 ratio in the infant mouse small intestine for 24 h. Data are presented as a single data point per mouse, along with median and interquartile range for each condition. Statistical analyses were performed using two-sided Mann-Whitney U test. P = 0.0003, 0.00008, 0.0813, 0.8329, and 0.0016 for the indicated pairs. In both assays, competitive indices (CI) were calculated as the ratio of output to input of the mutant strain relative to the WT. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns not significant.

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