Fig. 2: Prioritization of SPR fragment screening hits based on binding profile.
From: WRN structural flexibility showcased through fragment-based lead discovery of inhibitors
The prioritization of fragment hits was carried out by clustering each test fragment based on binding sensorgram characteristics. Each compound cluster was ranked as follows: a Rank 1, fast-on, fast-off binding kinetics; b Rank 2, biphasic binding profile with predominantly fast-on, fast-off binding and a minor component (roughly ≤ 20% of the binding profile) of slow-on, slow-off binding kinetics; c Rank 3, significant (> 20% of the binding profile) slow kinetic profiles; d Rank 4, super-stoichiometric/non-saturable binding.
