Fig. 2: Overexpression of A2AR selectively in the choroid plexus (ChP) (A2AR-OE) leads to cerebrospinal fluid (CSF) hypersecretion and enlarged ventricles.
A Scheme of the experimental design for exploring the impact of A2AR overexpression in the ChP using fMRI. B Representative immunofluorescence images of HA-tag (labeling A2AR) or EGFP in brain sections transduced with AAV2/5-A2AR-OE or control virus on day 14 following ICV viral injection. C Quantitative analysis of A2AR fluorescence intensity as shown in (B) (n = 5/group). D Representative immunoblots comparing ChP lysates from AAV-control vs. AAV-A2AR-OE mice (14 days after ICV-viral injection). E Quantification of A2AR protein levels from the Western blots illustrated in (D) (n = 5/group). F Representative fMRI images showing brain ventricles, obtained 14 days after induction of A2AR overexpression in the ChP. G Ventricle volumes of mice in control vs. A2AR-OE mice (control n = 10, A2AR-OE n = 8). H Brain sizes of mice in control vs. A2AR-OE mice (control n = 10, A2AR-OE n = 8). I Representative DAPI-stained images showing lateral ventricles after 14 days of ChP-targeted A2AR overexpression (n = 5/group). J Quantitative analysis of ventricular size as shown in (I) (n = 5/group). K Representative measurement of the CSF content of mice in the control vs. A2AR-OE group. L Quantitative analysis of CSF content as shown in (K) (n = 5/group). M Representative of flow trajectories of Evans blue dye (injected into the lateral ventricles) in control and A2AR-OE mice. Unpaired two-tailed Student’s t test. ns: non-significant. Data are mean ± s.e.m. Source data are provided as a Source Data file.
