Fig. 6: Compositional elemental analysis of p62-encapsulated lipid droplets using energy dispersive X-ray (EDX) spectroscopy. | Nature Communications

Fig. 6: Compositional elemental analysis of p62-encapsulated lipid droplets using energy dispersive X-ray (EDX) spectroscopy.

From: Structural organization of p62 filaments and the cellular ultrastructure of calcium-rich p62-enwrapped lipid droplet cargo

Fig. 6

a Scanning electron microscopy (SEM) image of a milled lamella with an inset of characterized lipid droplet. b Correlated SEM image superimposed with a reflected light channel and fluorescent channels of 595 nm for mCherry-p62 and 515 nm for autofluorescence. c Transmission electron microscopy image of the corresponding lamella. d Enlarged image of p62-positive lipid droplet. e High-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) image of a droplet of interest with corresponding EDX signals for Carbon in red (C), Oxygen in blue (O), Calcium in yellow (Ca), Nitrogen in green (N), Phosphorus in cyan (P) and Magnesium in purple (Mg). Scale bar 500 nm. Negative stain EM images of (f) purified p62 filaments, (g) with 100 µM CaCl2 and (h) 100 mM CaCl2. i Model summary of analyzed p62 droplet structures: the lipid droplet devoid of any phospholipids is encapsulated by a p62-positive layer that is rich in CaPO4.Experiments were done in one session, on 4 independent cellular lamellae and yielded similar results.

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