Fig. 2: AE1 in complex with PIP2 and PC.
A Model of AE1 dimer as viewed from inside the cell. AE1 protomers are colored in orange (IF1) and light green (OF), with PIP2 molecules in pink. B Close-up view of the AE1 dimer interface. PIP2 (pink) sits in the middle of the site. The key residues that mediate the interactions with the lipid at the interfaces are shown in stick representation. C Model of AE1 dimer as viewed in the plane of the membrane. PC lipids are shown in purple. D Close-up view of the interactions between AE1 protomers and a PC lipid (purple). The key residues that mediate the interactions are shown in stick representation. E, F 1-min uptake of 10 µM NaH[14C]O3 or Na[125I] by AE1-containing proteoliposomes. AE1 was incubated for 1 h with 15 mg/mL poly-lysine (Poly-Lys), 0.3 U/mL PLC before being incorporated into proteoliposomes. In F, 50 µM PIP2 was added to liposomes previously treated with PLC. Data in panels E and F are shown as mean value ± SEM from ≥ 6 biological replicates as indicated for each data set. The normality of the biological replicates was tested with the Shapiro–Wilk test, and a two-tailed Mann–Whitney U test was applied to non-parametric data, and a two-tailed unpaired Student’s t-test was used for parametric data.
