Fig. 5: Steady-state H2S and persulfides in rodent tissues in vivo. | Nature Communications

Fig. 5: Steady-state H2S and persulfides in rodent tissues in vivo.

From: Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H2S in biological samples

Fig. 5: Steady-state H2S and persulfides in rodent tissues in vivo.

a Tissues were lysed in the presence of IAM-TPP, divided into three and processed as indicated. b H2S detected as TPP-CAM-S-CAM-TPP. c Total persulfides detected as TPP-CAM-S-CAM. d LMW persulfides detected as TPP-CAM-S-CAM. e Protein persulfides quantified as (c, d). f Representative SAM analysis of mouse liver homogenate incubated with IAM-TPP, spiked with GSS-CAM-TPP and processed to quantify GSS-CAM-TPP. The x-axis intercept is the GSS-CAM-TPP concentration of the unspiked sample. g The experiment in (f) was repeated on two biological replicates and normalised to tissue weight to quantify GSS-CAM-TPP. Data are means ± SD, n = 3 (n denotes the number of individual biological replicates). Selected protein structure elements in (a) were prepared with the assistance of ChemDraw software (version 23.1.2). Source data are provided as a Source Data file.

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