Fig. 2: Temporal correlation of hairpin unwinding and 30S head rotation.
From: The ribosome derives the energy to translocate and unwind mRNA from EF-G binding
a Schematic of the fleezers hairpin unwinding setup with FRET for simultaneous detection of mRNA hairpin unwinding and ribosomal 30S head rotation during translocation. b Expected pattern of donor and acceptor fluorescence if hairpin unwinding coincides with forward head rotation. c A fleezers trajectory showing consecutive unwinding steps (first panel), anticorrelated fluorescence in the green and red channels (second panel), calculated ratiometric FRET efficiency (third panel), and FRET visualized by color (last panel, see “Methods”). d Filtered event average from many steps (n = 396) showing red-to-green (high-to-low FRET) transition around the time of hairpin unwinding. Source data are provided as a Source Data file.
