Fig. 4: MCL1 modulates HK2 in mTORC1-dependent manner.
From: MCL1 modulates mTORC1 signaling to promote bioenergetics and tumorigenesis
A Relative mRNA expression levels (normalized to beta-actin) of an array of metabolic regulators assessed by real-time qPCR in CHL-1 cells transduced with the indicated shRNAs and treated with or without Rapamycin (50 nM) for 24 h (n = 3 biologically independent samples). Data is presented as mean +/- SEM and significance is determined by paired two-tailed t-test. B Immunoblotting analysis of total cell lysates from CHL-1 cells treated as in (A) showing modulation of HK2 protein level by MCL1-mTORC1 axis. The samples derived from the same experiment but different gels for HK2, S6K1, MCL1, another for TSC2, pS6K1, S6 and another for pS6 were processed in parallel (C) Immunoblotting analysis of lysates of CHL-1 cells transduced with either scrambled shRNA or shRNA against MCL1, Bcl-2 or Bcl-xL showing specific modulation of HK2 levels by MCL1. The samples derived from the same experiment but different gels for HK2, pS6K1, MCL1, Bcl-xL, another for pS6, LDHA, another for S6K1, Bcl-2, and another for S6 were processed in parallel.
