Fig. 3: Knockdown of SCRIB affects ABCC4 function and membrane localization.
From: Protein Kinase A and assembly of an ABCC4 protein network
DAOY cells were transfected with 25 nM of siRNAs (h1 and h7) targeting human SCRIB or a negative control siRNA. a Representative immunoblots from 3 independent surface labeling experiments suggest that SCRIB suppression markedly reduced the level of ABCC4 in the plasma membrane. Bars (right) represent mean ± SD of surface ABCC4 level normalized to surface marker ATP1A1 from 3 biological replicates. **p < 0.01, one-way ANOVA. p-value = 0.0086 and 0.0040 for h1 and h7, respectively. b Cells were treated with different concentrations of Bis-POM-PMEA (0–10 µM) for 48 h, and cell viability was measured using the CTG assay. Mean ± SEM of assessed cell viability (%) from 3 biological replicates is shown.
