Fig. 5: Characterization of the filament-associated hair-like layer. | Nature Communications

Fig. 5: Characterization of the filament-associated hair-like layer.

From: Segmented filamentous bacteria undergo a structural transition at their adhesive tip during unicellular to filament development

Fig. 5

Close-ups of the tip side of: a/c(i) mouse-SFB and b/d(i) rat-SFB from reconstructed tomograms EMD-52677 and EMD-52699, respectively. The regions used for hair-like layer (HLL) analysis are delimitated by red rectangles. Regions containing: a/b(i) the inner part of the hair-like layer (I-HLL) and c/d(i) the outer part of the hair-like layer (O-HLL) shown in (a–d(ii)). The average spacing and standard deviation between HLL subunits is shown. a–d(iii), Fast Fourier transform, a–d(iv) Maximum intensity peaks (indicated by white arrow heads) and their corresponding frequency, and a–d(v) Plot profiles are shown for: a(i) mouse-SFB I-HLL, b(i) rat-SFB I-HLL, c(i) mouse-SFB O-HLL, d(i) rat-SFB O-HLL. e Schematic representation of inner and outer parts of the HLL. OW outer wall zone; IW inner wall zone. f Distance between HLL and Surface layer (S-layer, SL) subunits in mouse-SFB and rat-SFB. Ten measurements were performed for each of the 5 SFB from 2 independent experiments (n = 50 measurements). The distance between S-layer subunits are also shown in Fig. 1k. The mean is shown and the statistical significance was assessed using the One-way ANOVA (****: p < 0.0001, ns: not significant). The most important comparisons are shown. Source data are provided as a Source Data file. mSFB mouse-SFB; rSFB rat-SFB; OW outer wall zone; IW inner wall zone. Scale bars: a–d(i): 20 nm.

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