Fig. 2: H2A.Z incorporation at TSSs of developmental genes and CGIs.
From: Major waves of H2A.Z incorporation during mouse oogenesis and preimplantation embryo development
a H2A.Z ChIP-seq signal in different developmental stages at unique TSSs ±1 kbp (n = 32,166), clustered based on H2A.Z signal. Blast., blastocyst. b RNA levels31 at unique TSSs ±500 bp (n = 32,166), clusters based on Fig. 2a. c Bubble plots showing overlap between TSS clusters and oocyte and embryonic gene categories compared to an average distribution across all clusters. p-values were calculated by two-sided Chi-Square tests, Benjamini-Hochberg adjusted for multiple testing. Bubbles corresponding to a p-value < 0.05 were excluded from the plot. ZGA, zygotic genome activation; MGA, mid-preimplantation gene activation. d Heatmaps showing H2A.Z, CGIs, Gene bodies, G/C, and CpG densities at unique TSSs ±25 kbp (n = 32,166) sorted according to increasing relative position of the nearest CGI. e H2A.Z in different developmental stages at unique TSSs (n = 32,166), either overlapping (black) or not overlapping (blue) CGIs. Blast., blastocyst.
