Fig. 3: KDM1A overexpression improves cardiac dysfunction and GSH imbalance in CME rats in vivo. | Nature Communications

Fig. 3: KDM1A overexpression improves cardiac dysfunction and GSH imbalance in CME rats in vivo.

From: USP16 S-nitrosylation aggravates coronary microembolization-induced myocardial injury via repressing KDM1A-mediated glutathione homeostasis

Fig. 3: KDM1A overexpression improves cardiac dysfunction and GSH imbalance in CME rats in vivo.

A KDM1A expression in myocardial tissues was evaluated using immunohistochemical staining (scale bar = 100, 50 μm). P < 0.0001. B Cardiac function was determined using echocardiography. Detailed P values see (B). C Serum CK-MB level was measured using ELISA. Detailed P values see (C). D HBFP staining analyzed the microinfarct size in the hearts (scale bar = 100, 50 μm). The black arrow indicates a microsphere. Detailed P values see (D). E The pathological alterations in the myocardial tissues were observed by HE staining (scale bar = 100, 50 μm). The black arrow indicates a microsphere, and the red arrow indicates inflammatory cell infiltration. Detailed P values see (E). F Apoptosis in the myocardial tissues was assessed using TUNEL (scale bar = 100, 50 μm). Detailed P values see (F). G The expression of KDM1A, GCLM, and GLS in the myocardial tissues was measured using immunohistochemical staining (scale bar = 100, 50 μm). Detailed P values see (G). H The GSH/GSSG ratio in the heart tissues was calculated. Detailed P values see (H). I DCFH-DA staining detected the ROS level in the myocardial sections. Detailed P values see (I). Data are presented as mean ± standard deviation (SD). n = 9 rats per group, indicating nine biological repetitions. In A, B, D–G, I, experiments were repeated independently at least 9 times with similar results. Representative images from one experiment are shown. Unpaired two-tailed Student’s t-test (for A) and one-way ANOVA followed by Tukey’s test for multiple group comparison (for B–I) were performed to analyze data. Source data are provided as a Source data file. KDM1A lysine-specific histone demethylase 1A, GSH glutathione, CME coronary microembolization, CK-MB creatine kinase-MB, ELISA enzyme-linked immunosorbent assay, HE Hematoxylin-Eosin, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling, GCLM glutamate-cysteine ligase modifier subunit, GLS glutaminase, GSSG oxidized glutathione, ROS reactive oxygen species.

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