Fig. 3: DDX6 promotes AML cell growth and LSC self-renewal while suppressing myeloid differentiation.
From: DDX6 undergoes phase separation to modulate metabolic plasticity and chemoresistance
a Schematic outline of MLL-AF9-driven malignant transformation of Lin- HSCs. Created in BioRender. Bi, H. (2025) https://BioRender.com/ijytw0r. b Ddx6 KO efficacy in MLL-AF9-transduced murine Lin- HSCs. c Effect of Ddx6 KO on the colony-forming ability of MLL-AF9-transduced Lin- HSCs. d Representative images of colonies from MLL-AF9-transduced Lin- HSCs following Ddx6 KO. e Effect of DDX6 KO on the colony-forming ability of PDX cells. f DDX6 KO and OE efficacy in Mono-mac-6 cells as determined by Western blotting. g–i Effects of DDX6 KO and OE on the growth of THP-1 (g), Mono-mac-6 (h), and MA9.3RAS (i) cells. MTT assay was used. j Effects of DDX6 KO and OE on myeloid differentiation of MA9.3RAS cells. Flow cytometry was used. k, l Effects of DDX6 KO on myeloid differentiation of THP1 cells as determined by Wright-Giemsa staining (k) and flow cytometry (l). m Statistical results showing the effects of DDX6 KO on myeloid differentiation of THP-1 cells. n, o Effect of DDX6 KO and rescued expression with WT and truncated DDX6 on cell viability (n) and myeloid differentiation (o) in THP-1 cells. p DDX6 KO and OE efficacy in THP-1 as determined by Western blotting. q Effects of DDX6 KO and OE ΔIDR on the growth of Mono-mac-6 cells as assessed by MTT assay. r Effects of DDX6 KO and LSM14A OE on the growth of Mono-mac-6 cells. s Effects of DDX6 KO and LSM14A OE on the apoptosis of Mono-mac-6 cells as assessed by flow cytometry. t Effects of DDX6 KO and LSM14A OE on PBs number in Mono-mac-6 cells. sgDDX6-1 was utilized for (f, h–t). b, f, p: n = 3 independent experiments with consistent results; Data were represented as mean ± SEM (n = 5 for (e); n = 3 for (g–j, m–o, and q–s; independent experiments); Unpaired two-tailed Student’s t-test was utilized for all analysis. Source data are provided as a Source Data file.
