Fig. 4: Deletion of Ddx6 minimally impacts normal hematopoiesis.

a Schematic outline of the design of Ddx6 cKO mice. b Genotyping results of Ddx6 cKO mice. c Ddx6 KO efficacy in Lin^- HSCs as determined by Western blotting. d Schematic overview of poly (I:C)-induced Ddx6 cKO in mice. Created in BioRender. Bi, H. (2025) https://BioRender.com/qqp9hro. e–j Peripheral blood analysis of Ddx6 WT, heterozygous KO, and homozygous KO mice. The levels of WBC (e), LYM (f), PLT (g), RBC (h), HGB (i), and NEU (j) were displayed. k The percentage of Lin^-, LK, and LSK cells in the BM of Ddx6 WT, heterozygous KO, and homozygous KO mice. l Representative flow cytometric plots of LK and LSK cell populations in the BM of WT and Ddx6 KO mice. m Representative images of colonies in Lin^- HSCs following Ddx6 heterozygous and homozygous KO. n Effect of Ddx6 heterozygous and homozygous KO on the colony-forming ability of Lin^- HSCs. o Statistical results of PB numbers in Ddx6 WT and homozygous KO Lin^- HSC cells. p Frequencies of T-lymphoid (CD3⁺), B-lymphoid (B220⁺), myeloid (Mac1⁺Gr1⁺), and erythroid (Ter119⁺) cells in the spleen of Ddx6 WT, heterozygous KO, and homozygous KO mice. q Representative spleen images. Scale bar, 1 cm. r The spleen weight of Ddx6 WT, heterozygous KO, and homozygous KO mice. b, c: n = 3 independent experiments with consistent results; Data in (e–k, o, p, and r were presented as mean ± SEM (n = 5 WT mice, n = 5 heterozygous mice, n = 5 homozygous mice); Unpaired two-tailed Student’s t-test was utilized for all the statistical analysis. Source data are provided as a Source Data file.